2.0 ng mL detection limit competition method test principle 25OH Vitamin D

Indication
Vitamin D is a fat-soluble steroid hormone precursor that is mainly produced in the skin by exposure to sunlight. Vitamin D is biologically
inert and must undergo hydroxylation steps to become active.1 Our body can only synthesize vitamin D3. Vitamin D2 is taken up with
fortified food or given by supplements. Physiologically, vitamin D3 and D2 are bound to the vitamin D-binding protein (VDBP) in plasma
and transported to the liver to become 25-hydroxyvitamin D (vitamin D (25-OH)). As vitamin D (25-OH) represents the major storage form,
its blood concentration is used to assess the overall vitamin D status. More than 95 % of vitamin D (25-OH), measurable in serum, is
vitamin D3 (25-OH) whereas vitamin D2 (25-OH) reaches measurable levels only in patients taking vitamin D2 supplements.1,2,3 Vitamin D is
essential for bone health. In children, severe deficiency leads to rickets. In elderly, the risk of falling has been attributed to vitamin D
deficiency due to muscle weakness. Moreover, low vitamin D (25-OH) concentrations are associated with lower bone mineral density.
Insufficiency has also been linked to diabetes, cancer, cardiovascular disease, and autoimmune diseases.1 The DiaSino 25-OH Vitamin D is
intended for the quantitative determination of vitamin D (25-OH) in human serum, plasma, tissue homogenates and other biological fluids,
as an aid in the assessment of vitamin D sufficiency. Testing time85 minutesTest principleCompetition methodCalibrators0, 7.5, 15, 30, 75, 150 ng/mLSample materialSerumSample volume50 µLDetection limit2.0 ng/mLMeasuring range2-150 ng/mLTraceabilityRoche Elecsys®25OH Vitamin D total and internal serum panelExpected valuesMost experts agree that vitamin D deficiency should be defined as vitamin D
(25-OH) of ≤ 20 ng/mL. Vitamin D insufficiency is recognized as 21-29 ng/mL.
The preferred level for vitamin D (25-OH) is recommended to be ≥ 30 ng/mL.4,5