Elisa testing hiv test kit

HIV elisa test kit is for the detection of circulating antibodies to Human Immunodeficiency Virus Type 1 (HIV-1) and/or Human Immunodeficiency Virus Type 2 (HIV-2) in Human serum or plasma and is indicated as a screening test for serum or plasma and as an aid in the diagnosis of potential infection with HIV-1 and/or HIV-2.The HIV-1/HIV-2 EIA utilizes a detection system where microplate wells are coated with synthetic peptides and recombinant antigen corresponding to a highly antigenic segment of HIV-1/HIV-2 envelope and core proteins. Serum or plasma specimens, controls are added to the wells. During incubation, antibodies specific for HIV-1 and HIV-2 present in the specimen will bind to the peptides and recombinant antigen fixed onto the micro plate wells. The wells are washed to remove unbound materials, and recombinant antigen conjugate will bind to the antigen-antibody complex and excess unbound enzyme conjugates are again removed by washing. The enzyme substrate, tetramethylbenzidine (TMB), is added upon incubation the substrate will be hydrolyzed by the bound enzyme and a blue or blue-green color develops in wells containing HIV-1 and/or HIV-2 specific antibodies. The enzyme reaction is stopped by the addition of sulphuric acid. The intensity of color developed is read spectrophotometrically at 450nm(450 nm/630 nm) and is proportional to the amount of antibodies present in the specimen.Serum should be prepared from a whole blood specimen obtained by acceptable medical techniques. Either serum or plasma can be used in this test. Remove serum or plasma from the clot or blood cells as soon as possible to avoid hemolysis. Specimen with extensive particulate should be clarified by centrifugation prior to use. Specimen frozen at -20°C or colder may be used. Avoid repeated freeze thaw. STORAGE OF TEST KIT Unopened test kits should be stored at 2-8C. . The microtiter plate should be kept in a sealed bag to minimize exposure to damp air. Use up the reagents as soon as possible after the kit is unpacked.
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1:Factory direct price .price is competitive with high quality.
2: Original manufacturer provide OEM service for you.
3: Smooth delivery near beijing and tianjin Port.
4: Free samples sent for your evalutaiton.our advantage
1: Experience :We have 10 years of experience in research, delevopment, manufacturing and distributing advanced medical instruments and in -vitro medical diagnostic products.
2: Professionalism: All our research staffs are with professional, and have great achievements in medical diagnostic items
3: Quality: Our products are with ISO9001,ISO13485 GMP
4: Service: We offer you series of service, including pro sale and after sale service.We treat customers as friends. Fast reply via email,phone, Skype etc.
5: Price: We control our prodcution cost strictly, so you can get first -hand prices from our factory,
6: Delivery time: Fast delivery for you. 10~21 days after payment receipt