Selling high quality products in 2020 HCV Ab ELISA Kit

This is an enzyme linked immunosorbent assay using recombinant proteins derived from core regions of HCV virus to detect the presence of HCV antibodies in human sera.Multiple epitomes’ of HCV proteins (Core, NS3, NS4 and NS5) are bound to the micro titer wells. When antibodies to HCV are present in the test sample, they react with recombinant proteins and attach to the solid-phase. Non-reactive antibodies are removed with the wash buffer. Human IgGs bound to the antigen are reacted with anti-human IgG peroxidase conjugate and visualized by subsequent reactions with a chromogenic substrate. Positive sample generates a medium to dark blue color. No color or very pale blue color indicates a negative reaction. The intensity of the reaction is photo metrically quantities.DescriptionCoating PlateNegative ControlPositive ControlSample DiluentsEnzyme ConjugateWash Buffer Concentrate (20x)Substrate Solution ASubstrate Solution BStop SolutionPlastic BagSeal PaperManualAll human source material used in the preparation of this product was found to be negative for the presence of HCV antibodies, as well as for the hepatitis B surface antigen, using a commercial licensed method. Nevertheless, because no test method can offer complete assurance of the absence of infectious agents, this product should be handled with caution.Avoid contact of reagents with the eyes and skin. If that occurs, wash thoroughly with water.Wear gloves.Do not pipette by mouth.Do not smoke.Dispose all used materials in a suitable biohazard us waste container. Remains of samples, controls, aspirated reagents and pipette tips should be collected in a container for this purpose and autoclaved 1-hour at 121°C or treated with 10% sodium hypochlorite (final concentration) for 30 min before disposal. (Remains containing acid must be neutralized prior addition of sodium hypochlorite).Adjust washer to the plate used (flat bottom) in order to wash properly.Do not mix reagents from different lots.Do not use reagents after expiration date.Extreme care should be taken to avoid microbial contamination and cross contamination of reagents.Use a new pipette tip for each specimen and each reagent.Soaps and/or oxidizing agents remaining in containers used for the substrate-TMB solution can interfere with the reaction.Serum should be prepared from a whole blood specimen obtained by acceptable medical techniques. Either serum or plasma can be used in this test. Remove serum or plasma from the clot or blood cells as soon as possible to avoid hemolytic. Specimen with extensive particulate should be clarified by centrifugation prior to use. Specimen frozen at -20°C or colder may be used. Avoid repeated freeze thaw. STORAGE OF TEST KIT
Unopened test kits should be stored at 2-8C upon receipt and the micro titer plate should be kept in a sealed bag to minimize exposure to damp air. Use up the reagents as soon as possible after the kit is unpacked.It is strongly advised to analyze each specimen and controls in duplicate. All the reagents should equilibrate to room temperature before use.Dispense 100ml(or 3 drops) of specimen diluents into individualtest wells.Dispense 100ml positive control and negative control duplicate into individual wells.Add 10ml of each test sample into duplicate test wells; vortex to mix.Incubate for 60 minutes at 37°CWash each well 5 times by filling each well with diluted wash buffer, then inverting the plate vigorously to get all water out and blocking the rim of wells on absorbent paper for a few seconds.Add 100ml of Enzyme Conjugate to each well. Mix it gently by swirling the micro titer plate on flat bench for 1 minute. Do not add Enzyme Conjugate to the blank well.Incubate for 30 minutes at 37°CWash the plate 5 times as step 5.Add one drop (50ml) of Substrate Solution A (HRP-substrate) to each well, then add one drop (50ml) of Substrate Solution B (TMB) to each well. Mix gently and incubate at 37°C for 30 minutes. .