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blood testing reagent HBV Hepatitis B virus HBsAb ELISA Kit

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50 Box	US $6.94/ Box

Local Area:	Beijing, China
R&D Capacity:	OEM, ODM, Other
Payment Terms:	LC, T/T, D/P, Paypal, Western Union

Brand:	bioneovan
Place of Origin:	Beijing, China
Brand Name:	bioneovan
Model Number:	Immunoassay test

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CertificateGMPSFDAISO13485ISO9001:2008
HBsAb ELISA kit
CatalogNo. NAMEPACKAGE SIZE:
BE102AHBsAb ELISA kit 96 tests/Kit
SUMMARY
For detection of anti-HBs, this kit uses antigen “sandwich” ELISA method where polystyrene microwell strips are pre-coated with recombinant HBsAg. Patient’s serum or plasma sample is added to the microwells together with a second HBsAg conjugated to Horseradish Peroxidase (HRP-Conjugate). In case of presence of anti-HBs in the sample, the pre-coated and conjugated antigens will be bound to the two variable domains of the antibody and during incubation, the specific immunocomplex formed is captured on the solid phase. After washing to remove sample serum proteins and unbound HRPConjugates, Chromogen solutions containing Tetramethylbenzidine (TMB) and urea peroxide are added to the wells. In presence of the antigen-antibody-antigen(HRP) “sandwich” complex, the colorless Chromogens are hydrolyzed by the bound HRP-Conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and is proportional to the amount of antibody captured in the wells, and to the sample respectively. Wells containing samples negative for anti-HBs remain colorless.
PRINCIPLE
This anti-HBs ELISA kit is an enzyme linked-immunosorbent assay for in vitro qualitative detection of antibodies to hepatitis B virus surface antigen (anti-HBs) in human serum or plasma. It is intended for use in medical laboratories for diagnosis and management of patients related to infection with hepatitis B virus. ANTIBODIES TO HEPATITIS B VIRUS CORE ANTIGEN ELISA. One-Step Incubation, Double Antigen Sandwich Principle
Item
Description
96T
480T
1
Micro titer Well
1
5
2
Negative Control
0.5ml
2.5ml
3
Positive Control
0.5ml
2.5ml
4
Enzyme Conjugate
6ml
30ml
5
Wash Buffer Concentrate (20x)
20ml
100ml
6
Substrate Solution A
6ml
30ml
7
Substrate Solution B
6ml
30ml
8
Stop Solution
6ml
30ml
9
User manual
1 copy
5 copy