elisa antibody hepatitis c test kit hcv

Hepatitis C virus (HCV), which was formerly described as the parent rally transmitted form of non-A, non-B hepatitis (NANBH)1, becomes a chronic disease in 50% of the cases.2HCV can also be transmitted through intravenous drug abuse, sexual , and household contact.3 Hepatitis C virus is a single stranded RNA virus with some structural relations to the flavivirus family. Nucleic acid sequences of HCV cDNA clones provided the basis for the construction of recombinant peptides representing putative hepatitis C virus proteins.4,5Anti-hepatitis C virus antibody screening of blood using synthetic or recombinant proteins, helped to identify apparently healthy blood donors with anti-HCV antibodies who otherwise might have transmitted the virus.6
This is an enzyme linked immunosorbent assay using recombinant proteins derived from core regions of HCV virus to detect the presence of HCV antibodies in human sera.Multiple epitomes’ of HCV proteins (Core, NS3, NS4 and NS5) are bound to the micro titer wells. When antibodies to HCV are present in the test sample, they react with recombinant proteins and attach to the solid-phase. Non-reactive antibodies are removed with the wash buffer. Human IgGs bound to the antigen are reacted with anti-human IgG peroxidase conjugate and visualized by subsequent reactions with a chromogenic substrate. Positive sample generates a medium to dark blue color. No color or very pale blue color indicates a negative reaction. The intensity of the reaction is photo metrically quantities.Description
96T
1
Coating Plate
1
2
Negative Control
1ml
3
Positive Control
1ml
4
Sample Diluents
12ml
5
Enzyme Conjugate
12ml
6
Wash Buffer Concentrate (20x)
30ml
7
Substrate Solution A
6ml
8
Substrate Solution B
6ml
9
Stop Solution
6ml
10
Plastic Bag
1
11
Seal Paper
3
12
Manual
1 copyAll human source material used in the preparation of this product was found to be negative for the presence of HCV antibodies, as well as for the hepatitis B surface antigen, using a commercial licensed method. Nevertheless, because no test method can offer complete assurance of the absence of infectious agents, this product should be handled with caution.
Avoid contact of reagents with the eyes and skin. If that occurs, wash thoroughly with water.Wear gloves.Do not pipette by mouth.Do not smoke.Dispose all used materials in a suitable biohazard us waste container. Remains of samples, controls, aspirated reagents and pipette tips should be collected in a container for this purpose and autoclaved 1-hour at 121°C or treated with 10% sodium hypochlorite (final concentration) for 30 min before disposal. (Remains containing acid must be neutralized prior addition of sodium hypochlorite).Adjust washer to the plate used (flat bottom) in order to wash properly.Do not mix reagents from different lots.Do not use reagents after expiration date.Extreme care should be taken to avoid microbial contamination and cross contamination of reagents.Use a new pipette tip for each specimen and each reagent.Soaps and/or oxidizing agents remaining in containers used for the substrate-TMB solution can interfere with the reaction.
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