ASA Enzyme-linked Immunosorbent Assay (ELISA) Test Kit ASA Elisa TestSpecifications
1. Elisa test kits
2. Packing: 96test/box
3. specimen: serum/plasma
4. ISO13285, ISO 9001
Product Description
Diagnostic Elisa Test Kits
Enzyme-linked Immunosorbent Assay
1. All reagents should be allowed to reach room temperature for 15 minutes before use.
2. Prepare 1:40 dilution of Wash Buffer Concentrate with distilled water.
3.Add Sample Diluent 100 ul (2 drops) into the appropriate wells except the blank well, negative
well (blank and positive well set 1well, negative well set 2 wells).
4. Add 10ul specimen to the well, beating by pipet repeatedly until liquid turn blue,
Dispense 50ul negative and positive control to the negative and positive well separately, do not dispense liquid to blank control well.
5. Flick the microtiter wells for 30 seconds and mix well. Affix to sealing template, Incubate at 37 for 20 minutes.
6. Take out, Add Wash Buffer to each well and absorb after 20 seconds. Repeat 5 times until each well is dry.
7. Dispense 1 drop(50ul) of HRP Conjugate to each well except the blank well.Gently vibration mixture, mix well, Affix to sealing template. Incubate at 37 for 20 minutes.
8. Add Wash Buffer to each well and absorb after 20 seconds. Repeat 5 times until each well is dry.
9. Dispense 1 drop(50ul) of Substrate A and 1 drop(30ul) of Substrate B into every well except blank well. Incubate at 37 for 10 minutes.Gently vibration mixture, mix well, Incubate at 37 for 10 minutes.
10.Take out, adding 1 drop(50ul) of Stop Solution except the blank well ,mix well. Read result with a microwell reader.
Read Results
1. Colorimetry: Read O.D at 450nm with a microwell reader.
Cut-off O.D
=2.1xNegative Control O.D. (If the O.D value of the Negative Control is lower than 0.09, calculate as per 0.09;if the O.D. value is more than 0.09,calculate as the actual data).
Positive: Sample O.D≥ Cut-off O.D.
Negative: Sample O.D<Cut-off O.D.
2. Ratio method:
Positive :Sample OD/Cut off (S/Co) ≥1
Negative : Sample OD/Cut off (S/Co) <1
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