Dgtp dntp pcr dna

Certificate of AnalysisProduct: dGTP, 100mM Solution
Lot No. 20081223Product Name
2-Deoxyguanosine-5-Triphosphate, Trisodium Salt
Molecular Formula
C10H13N5Na3O13P3
Molecular Weight
573.1
CAS Number
93919-41-6
TEST
SPECIFICATION
RESULTS
Appearance
Clear Colorless solution
passed
Concentration
100mM±5 mM
100.2
Purity (HPLC)
≥99%
99
λmax
å = 13.7x103 M-1cm-1 at pH 7.0)
253nm±2nm
1.373(x1000)
Absorbance Rations
A250/A260 1.15±0.03
1.16
(pH 7.0)
A280/A260 0.67±0.03
0.69
pH (22~25)
7.0-7.5
7.4
Quality control
Molecular Biology Grade
1. The concentration is verified by optical density spectrophotometry.
2. This preparation is free of DNase and RNase contamination as determined by by agarose gel.
3. HPLC (column C18; detection UV at 253; mobile phase: A=TEAA0.1M, pH 7.0; B=60% CH3CN/A).5. Nickase is none detected by agarose gel.
Storage Recommendations: Always aviod multiple freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.
Long Term (Infrequent use; 1-2 times per month): -70
Daily/Weekly use: -20
Description

dNTP Set consits of 100mM aqueous solutions at pH 7.0 of each of dATP, dCTP, dGTP and dTTP, each in a separate vial. Because the nucleotides are provided in separate solutions, dNTP sets offer maximum flexibility in preparation of reaction mixes for different applications.
Applications
For use in all molecular biology applications, including PCR, long PCR, real-time PCR, high fidelity PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing and DNA labeling.
Quality ControlFunctionally tested in PCR with Taq and Pfu DNA Polymerases. Greater than 99% purity of each component confirmed by HPLC. Free of endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease and phosphatase activities
Storage

Store at -20°C.
Supply OEM and Bulk service
OEM:0.1ml-50ml
Bulk:50ml-5L