SYBRGreen I nucleic acid gel stain
10,000x in DMSO
usage
1.0mL sufficient for 100mini-gels
greener alternative product characteristics
Designing Safer Chemicals
concentration
10,000x in DMSO
storage temp.
−20°C
Description
SYBR is a registered trademark of Life Technologies
Application
A proprietary unsymmetrical cyanine dye which is an ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels. SYBR Green I can also be used to detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any prewashing steps, although the sensitivity is lower (approximately 100 to 300 pg per band using 254 nm epi-illumination). SYBR Green II is recommended for ss-DNA and RNA.SYBR Green I stain has been shown to be much less mutagenic than ethidium bromide in Ames tests. SYBR Green I is also a very sensitive stain for oligonucleotides, allowing for detection of as little as 1-2 ng of a synthetic 24-mer on a 5% polyacrylamide gel, which is 50-100 times greater sensitivity than obtained with ethidium bromide. Staining agarose gels with SYBR Green I does not interfere with the transfer of nucleic acids to membranes or subsequent hybridization in Southern or Northern blot analysis as long as 0.1%-0.3% SDS is included in prehybridization and hybridization buffers to remove the dye.
BEIJING SOLARBIO SCIENCE & TECHNOLOGY CO.,LTD
With our responsible sense and excellent products, we are ready to provide customers with first-class products and reliable experimental protocols. Our core spirit is "focusing on our customers, backing on our products, basing on credit, and aiming at innovation". Our goal is to provide the most academic and excellent service with our rich experience, excellent quality products at competitive prices, abundant stockpiles, and exact delivery schedules.
Tel:+86-18733521706