Taq DNA Polymerase

Taq DNA Polymerase
Cat No.: PC1100
Enzyme Activity: 5U/μl
Package: 500U/1000U/2500U, 10xPCR Buffer (with MgCl2).
Storage: at -20℃ for more than one year.
Product Description
Taq DNA Polymerase is purified fromexpressing a cloned DNA polymerase fromThe enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TaqDNA polymerase has 5′ to 3′ DNA polymerase activity and5′to 3′exonuclease activity lacking 3′-5′exonuclease activity.
In PCR reaction the extension rate is about 1-2 kb/min. Template-independent “A” can be generated at the 3’ end of the PCR product. It can be used in PCR for TA-Vector cloning, PCR amplification of genomic DNA, DNA sequencing, DNA labeling.
Unit Definition
One unit (U) is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble material in 30 minutes at 74°C, with activated salmon sperm DNA used as template.
Application
Taq DNA Polymerase has higher efficiency but lower fidelity than pfu DNA Polymerase. It is often used to amplify no more than 6 kb fragments. The product can be used for TA-Vector cloning directly.
Quality Control:99% purity as estimated by HPLC. The nucleotide is tested in PCR reactions.
Storage buffer: 20mM Tris-HCl (pH 8.0); 0.1mM EDTA; 1mM DTT; 100mM KCl; 50% glycerol; GC enhancer.
Reaction Components (50μl Volume)
Component
Volume
Template
<0.5μg
Forward Primer (10 μM)
1μl
Reverse Primer (10 μM)
1μl
10xBuffer(with Mg2+)
5μl
dNTP( 2.5mM each)
4μl
Taq DNA Polymerase
0.5-1μl
ddH2O
up to 50μl